Unveiling the role of ferrous ion in driving microalgae granulation from salt-tolerant strains for mariculture wastewater treatment.

Development of microalgal-bacterial granular sludge (MBGS) from saline-adapted microalgae is a promising approach for efficient mariculture wastewater treatment, whereas the elusive mechanisms governing granulation have impeded its widespread adoption. In this study, spherical and regular MBGS were successfully developed from mixed culture of pure Spirulina platensis and Chlorella sp. GY-H4 at 10 mg/L Fe2+ concentration. The addition of Fe2+ was proven to induce the formation of Fe-precipitates which served as nucleation sites for microbial attachment and granulation initiation. Additionally, Fe2+ increased the prevalence of exopolysaccharide-producing cyanobacteria, i.e. Synechocystis and Leptolyngbya, facilitating microbial cell adhesion. Furthermore, it stimulated the secretion of extracellular proteins (particularly tryptophan and aromatic proteins), which acted as structural backbone for the development of spherical granule form microalgal flocs. Lastly, it fostered the accumulation of exogenous heterotrophic functional genera, resulting in the efficient removal of DOC (98 %), PO43--P (98 %) and NH4+-N (87 %). Nevertheless, inadequate Fe2+ hindered microalgal floc transformation into granules, excessive Fe2+ expanded the anaerobic zone within the granules, almost halved protein content in the TB-EPS, and inhibited the functional genes expression, ultimately leading to an irregular granular morphology and diminished nutrient removal. This research provides valuable insights into the mechanisms by which Fe2+ promotes the granulation of salt-tolerant microalgae, offering guidance for the establishment and stable operation of MBGS systems in mariculture wastewater treatment.

Current Progress, Challenges and Perspectives in the Microalgal-Bacterial Aerobic Granular Sludge Process: A Review.

Traditional wastewater treatment technologies have become increasingly inefficient to meet the needs of low-consumption and sustainable wastewater treatment. Researchers are committed to seeking new wastewater treatment technologies, to reduce the pressure on the environment caused by resource shortages. Recently, a microalgal-bacterial granular sludge (MBGS) technology has attracted widespread attention due to its high efficiency wastewater treatment capacity, low energy consumption, low CO2 emissions, potentially high added values, and resource recovery capabilities. This review focused primarily on the following aspects of microalgal-bacterial granular sludge technology: (1) MBGS culture and maintenance operating parameters, (2) MBGS application in different wastewaters, (3) MBGS additional products: biofuels and bioproducts, (4) MBGS energy saving and consumption reduction: greenhouse gas emission reduction, and (5) challenges and prospects. The information in this review will help us better understand the current progress and future direction of the MBGS technology development. It is expected that this review will provide a sound theoretical basis for the practical applications of a MBGS technology in environmentally sustainable wastewater treatment, resource recovery, and system optimization.